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When making use of IEM, i5 sequences should be entered into these software during the ahead orientation in all situations and for all instrument sorts.

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Consult with Index two (i5) Orientation for more information on how to enter i5 bases within the sample sheet in ahead or reverse enhance orientation.

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When employing BaseSpace Sequence Hub PrepTab, i5 sequences needs to be entered into these computer software from the ahead orientation in all situations and for all instrument sorts.

For operates uploaded manually (i.e., an Illumina Resource is just not being used to produce the sample sheet), the index orientation relies on which instrument that you are utilizing. Please begin to see the Index Orientation Desk for information on tips on how to enter the i5 sequence depending upon the instrument and software package utilized.